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To compare network connectivity between idiopathic generalized epilepsy (IGE) and healthy controls and to evaluate interictal changes in IGE. A group of IGE patients (28) and age-matched healthy controls (30) were enrolled. Resting state EEG was performed for a 5-min period. By using a novel method based on ICA, we estimated topological parameters of the resting-state functional connectivity network. Then the differences of the parameters between IGE and controls were compared and the differences of the indices before and after antiepileptic drugs treatment (AEDs) were also compared. No significant differences in global connectivity and local connectivity were found. Between-group comparison revealed reduced local efficiency in IGE. However, interictal changes in IGE were not detectable by our method. The resting-state EEG network of IGE showed decreased local efficiency. This observation was not evident in a group of healthy controls. The interictal changes in IGE were undetectable using the current approach. An integrated approach to study the interictal changes in IGE is needed.Detection of early response of pig endothelial cells to TNF-alpha by time-lapse cytometry.
Endothelial cells (EC) of the porcine aorta, cultured in vitro, are putatively used for the study of inflammatory and stress effects on EC. In this study we investigated the interrelationships between the early response of porcine aortic EC to TNF-alpha by time-lapse cytometry (TL-Cyt). The TL-Cyt is a novel technique that synchronizes the recording process with the cell cycle. The cells were treated with 10 ng/ml of TNF-alpha and the kinetics of the cell cycle (G1, S, G2) was followed.
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